![]() ![]() This study helps to explain why genetic reduction in ASGR1 reduces cholesterol in humans and advances the concept that hepatic ASGR1 could be a therapeutic target in the liver for lowering of cholesterol and risk of ASCVD. Detection of ASGR1/ASGPR1 in HepG2 Human Cell Line by Flow Cytometry. Both a small interfering RNA (siRNA) and an antibody targeted to ASGR1 were found to lower cholesterol in mice through a similar mechanism. 4 now show that genetic deletion of Asgr1 in mice lowers serum and cholesterol and promotes cholesterol excretion through a mechanism involving stabilization of liver X receptor (LXR), without activation of sterol regulatory element binding protein (SREBP1) and lipogenesis. Genetic variants that reduce function of the asialoglycoprotein receptor (ASGR) found within the gene encoding its primary component ( ASGR1) have been reported to be associated with reduced levels of cholesterol and protection from coronary artery disease 3. The monoclonal antibody 8D7 is cross reactive with human ASGPR. The monoclonal antibody 8D7 recognizes a subunit-specific epitope of RHL-1 of rat ASGPR. The 8D7 monoclonal antibody recognizes Asialoglycoprotein receptor 1 (ASGPR 1), also known as Hepatic lectin H1 (HL-1). ASGR1 Antibody, PA5-32030, from Invitrogen. Interspecies Information Highest antigen sequence identity to the following orthologs: Mouse ENSMUSG00000020884 (76) Rat. Antibody validation: Validated for Immunofluorescence, Immunohistochemistry and Western Blot. Recombinant Mouse ASGPR1 Protein (Catalog50083-M07H) Preparation. Custom antibody services and bulk production also available. Our understanding of cholesterol metabolism has been substantially enhanced by discoveries in human genetics, which have led to several new therapeutic approaches to reducing blood cholesterol and risk of atherosclerotic cardiovascular disease (ASCVD) 1, 2. The rat asialoglycoprotein receptor (ASGPR) consists of three polypeptide subunits: rat hepatic lectin (RHL)-1, RHL-2 and RHL-3. Rabbit Polyclonal Anti-ASGR1 Antibody against Human asialoglycoprotein receptor 1. Anti-ASGR1 Rabbit Polyclonal Antibody (50083-T24), manufactured by Sino Biological is validated in ELISA,IHC-P. ![]()
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |